期刊
MOLECULAR AND CELLULAR PROBES
卷 39, 期 -, 页码 41-46出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2018.04.004
关键词
CPV-2; VP2 gene; nfo probe; RPA; Lateral flow strip
类别
资金
- Natural Science Foundation Youth Project of Hebei Province [C2017325001]
- Science and Technology Project Foundation of Hebei Province [16226604D]
- Biology Postdoctoral Science Foundation of Hebei Normal University [183717]
- Fund for One-hundred Outstanding Innovative Talents from Hebei Institution of Higher Learning [SLRC2017039]
A visible and equipment-free recombinase polymerase amplification assay combined with a lateral flow strip (LFS RPA) was developed to detect canine parvovirus type 2 (CPV-2), which is the etiological agent of canine parvovirus disease. The CPV-2 LFS RPA assay was developed based on the VP2 gene and is performed in a closed fist using body heat for 15 min; the products are visible to the naked eye on the LFS within 5 min. The assay could detect CPV 2a, CPV-2b and CPV-2c, and there was no cross-reaction with the other viruses tested. Using the standard CPV-2 DNA as a template, the analytical sensitivity was 1.0 x 10(2) copies per reaction, which was the same result as that of a real-time PCR. The assay performance was further evaluated by testing 60 canine fecal samples, and CPV-2 DNA was detected in 46 samples (76.7%, 46/60) by LFS RPA, which was the same result as that of the real-time PCR assay and higher than that of the SNAP method (48.3%, 29/60). The novel CPV-2 LFS RPA assay is an attractive and promising tool for rapid and convenient diagnosis of CPV disease, especially cage side and in underequipped laboratories.
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