期刊
MOLECULAR AND CELLULAR NEUROSCIENCE
卷 89, 期 -, 页码 71-79出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2018.04.005
关键词
DAMPs; Cytokines; Glial cells; Mac-1; Microglia; Neuroinflammation; Neurotoxicity; RAGE
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Jack Brown and Family Alzheimer's Disease Research Foundation
Neuroinflammation is a common pathogenic mechanism for a number of neurodegenerative disorders including Alzheimer's and Parkinson's diseases. Microglia, the immune cells of the brain, contribute to the onset and progression of the neuroinflammation observed in these diseases. Microglia become activated and initiate an inflammatory response by interacting with a diverse set of molecules, including the group of endogenous proteins released upon cell damage, termed damage-associated molecular patterns (DAMPs). One of these molecules, mitochondria] transcription factor A (TFAM), has been shown to induce pro-inflammatory and cytotoxic responses of microglia in vitro. Here, we demonstrate that TFAM injected into the cisterna magna of male Sprague-Dawley rats upregulates (i) the expression of monocyte chemotactic protein (MCP)-1, interleukin (IL)-1 beta, IL-6, tumor necrosis factor (TNF)-alpha and nuclear factor-kappa B inhibitor alpha (NF-kappa BIA) in the hippo campus; (ii) the expression of MCP-1, IL-1 beta and TNF-alpha in the frontal cortex; and (iii) protein concentration in both these brain regions. These same inflammatory mediators are upregulated in isolated rat microglia following their in vitro exposure to extracellular TFAM. Blocking the receptor for advanced glycation endproducts (RAGE) and the macrophage antigen complex (Mac)-1 by specific antibodies inhibited the TFAM-induced secretion of MCP-1 by THP-1 monocytic cells, which were used to model human microglia. Our data support the hypothesis that extracellular TFAM can interact with RAGE and Mac-1 to function as a DAMP that causes pro inflammatory microglial activation. Blocking this interaction may represent a potential target for attenuating the neuroinflammation observed in neurodegenerative diseases.
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