期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 472, 期 -, 页码 50-56出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2017.11.018
关键词
EBF1; microRNA; Adipocyte morphology; White adipose tissue
资金
- Swedish Research Council [DIA2016-097]
- Swedish Diabetes Foundation [171231 K2012-55X-01034-46-5]
- Diabetes Program
- CIMED Senior Investigator grants at Karolinska Institutet
- Tore Nilsson Foundation
- Foundation for Gamla Tjanarinnor
- Ake Wiberg Foundation
- EFSD/Lilly program
- Novo Nordisk foundation
- Tripartite Immuno-metabolism Consortium (TrIC) [NNF15CC0018486]
- MSAM consortium [NNF15SA0018346]
- European Foundation for the Study of Diabetes [Lilly 2014_5] Funding Source: researchfish
- Novo Nordisk Fonden [NNF15OC0015894, NNF15SA0018486, NNF15SA0018346, NNF14OC0010187] Funding Source: researchfish
Reduced adipose expression of the transcription factor Early B cell factor 1 (EBF1) is linked to white adipose tissue (WAT) hypertrophy. We aimed to identify microRNAs (miRNAs) associated with WAT hypertrophy and EBF1 regulation. We mapped WAT miRNA expression from 26 non-obese women discordant in WAT morphology and determined EBF1 activity in the non-obese and 30 obese women. Expression of 15 miRNAs was higher in hypertrophy and 10 were predicted to target EBF1. Binding of miR-365-5p/miR-574-5p were validated with 3'-UTR assay. Overexpression of miR-365-5p or miR-5745p reduced EBF1 while inhibition of miR-574 increased EBF1 expression in human adipocytes in vitro. Additive effects on EBF1 were observed when concomitantly overexpressing both miRNAs. EBF1 targets were affected by over expression/inhibition of either miRNAs. Finally, miR-365-5p/miR-574-5p expression in 56 individuals correlated significantly with EBF1 activity. Our results suggest that miR-365-5p and miR-574-5p may be linked to WAT hypertrophy via effects on EBF1 expression. (C) 2017 Elsevier B.V. All rights reserved.
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