期刊
MICROCHIMICA ACTA
卷 185, 期 7, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-2897-0
关键词
Gold nanoparticles; Test strip; Immunochromatography strip; Surface modification; Cetyltrimethylammonium bromide; Antibody; Food pathogen; Bacterial cell
资金
- National Natural Science Foundation of China [21675028, 21575027, 51473179, 21575025]
- Key Project of Fujian Province [2015Y0050]
- Youth Innovation Promotion Association of Chinese Academy of Sciences [2016268]
- Fujian Province-Chinese Academy of Sciences STS project [2017 T31010024]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R11]
Ligand exchange on the surface of gold nanorods (AuNRs) is widely used, but conventional methods usually require multiple centrifugation cycles to completely remove cetyltrimethylammonium bromide (CTAB). This can lead to undesired aggregation of AuNRs. A dialysis-assisted protocol is described here for ligand exchange on AuNRs. Dialysis driven by a concentration gradient is shown to be a powerful tool to separate CTAB from aqueous solutions. The concentration gradient of CTAB in a dialysis bag can avoid the possible aggregation of AuNRs that can be caused by drastic environmental changes. It also supports the rate of ligand exchange on the surfaces of the AuNRs. The modified AuNRs were employed in a lateral-flow test strip immunoassay (LFTS-IAs) for the food pathogen E. coli O157:H7 in order to study of efficiency of ligand exchange. Compared to AuNRs where ligand exchange was performed via multiple centrifugation cycles, the AuNRs prepared by dialysis-assisted ligand exchange show improved conjugation to antibody and enhanced visual signals in the test line of the LFTS-IAs. A portable strip reader (absorption wavelength = 525 nm) is used to records the testing results. The sensitivity of AuNRs modified by dialysis has been achieved even as low as 1 x 10(2) cfu.mL(-1) in a short time (within 15 min), and the working range is 1 x 10(2) to 1 x 10(6) cfu.mL(-1), which is superior over the detection performance of conventional test strip using AuNRs modified by centrifugation.
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