期刊
MICROCHIMICA ACTA
卷 185, 期 3, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-2726-5
关键词
Microfluidic system; Biomarker; Enhancement; Sandwich immunoassay; Nanoparticle; Horse radish peroxidase; Luminol
资金
- Shaanxi Province Science Foundation [2015JM2066]
- Fundamental Research Funds for the Central Universities [GK201603049, GK201701002]
- Program for Innovative Research Team in Shaanxi Province [2014KCT-28]
A paper-based chemiluminescence (CL) immunodevice is described for sensitive determination of the carcinoembryonic antigen (CEA). Capture antibody (Ab1) was first immobilized on the paper-based chip after plasma treatment of the paper surface. Next, carbon nanospheres functionalized with horse radish peroxidase (HRP) were attached as a signal antibody label. Strongly enhanced CL was achieved from the HRP catalyzed luminol/H2O2 CL system when using a sandwich immunoassay format. CEA can be quantified with a lower detection limit of 3 pg.mL(-1). The method displays an almost 10-fold increased sensitivity in comparison to a commercial Ab2-HRP based kit. Conceivably, this sensitive method may also be extended by designing sensitive assays for other biomarkers of interest in point-of-care diagnostics.
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