期刊
MICROCHIMICA ACTA
卷 185, 期 7, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-2886-3
关键词
Functionalized ionic liquid; Ferrocene; AuNP-dendrimer conjugate; Label-free immunosensor; alpha-Fetoprotein
资金
- Hunan Provincial Natural Science Foundation of China [2016JJ6105]
- Foundation of Hunan University of Arts and Science [17ZD03]
An immunosensor is described for the voltammetric determination of alpha-fetoprotein. It is making use of an AuNP-dendrimer conjugate and an ionic liquid. A gold electrode was first modified with chitosan. Then, the AuNP-dendrimer conjugate was covalently immobilized on the electrode. Following this, an ionic liquid was placed on the electrode via formation of a covalent bond between the amino groups of PAMAM and the aldehyde groups of an ionic liquid containing ferrocene. Thus, the redox probe ferrocene becomes immobilized on the electrode surface. PAMAM increases the amount of ferrocene immobilized on the electrode due to its globular shape and rich amino groups. The use of AuNPs improves the conductivity of the electrode. The modified electrode was applied to the determination of alpha-fetoprotein in human serum and has a linear response that covers the 0.05 to 30 ng mL(-1) alpha-fetoprotein concentration range, with a detection limit of 0.02 ng mL(-1). This assay is stable, selective and reproducible. It is perceived to provide a powerful tool for the early detection of cancer markers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据