期刊
MICROCHIMICA ACTA
卷 185, 期 6, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-2833-3
关键词
Exonuclease III; Nucleic acid detection; Colorimetric assay; DNA recycling; Amplification; Absorbance; Serum samples
资金
- Scientific Research Project of Beijing Educational Committee [KM201710028009]
The authors describe a colorimetric method for the determination of DNA based on the deaggregation of gold nanoparticles (AuNPs) induced by exonuclease III (Exo III). DNA amplification is accomplished by Exo III to generate large quantities of the residual DNA. Residual DNA tethers onto the surfaces of AuNPs which prevents their aggregation. Hence, the color of the solution is red. However, in the absence of DNA, salt-induced aggregation is not prevented, and the bluish-purple color of the aggregated AuNPs is observed. The ratio of absorbances at 525 and 625 nm increases up to 150 nM DNA concentrations, and the LOD is as low as 3.0 nM. It is shown that the presence of 300 nM concentrations of random DNA (with a mass up to 10-fold that of target DNA) does not interfere. The method was successfully applied to the analysis of DNA in spiked serum samples. The method is simple, reliable, and does not require complicated amplification steps and expensive instrumentation.
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