期刊
MICROCHIMICA ACTA
卷 185, 期 2, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-017-2657-6
关键词
Immunochromatographic strip; Sandwich immunoassay; Tetanus biosensor; Tetanus neurotoxin; Colorimetric detection; Human blood sample
资金
- National Natural Science Foundation of China [21205108, 21605038]
- Outstanding Young Talent Research Fund of Zhengzhou University [1421316038]
- Scientific and Technological Project of Henan Province, China [152102410006]
The authors describe a lateral flow assay (LFA) for the antibody against the infectious bacterium Clostridium tetani. Gold nanoparticles (AuNPs) were linked to tetanus antigen and are captured in the test line via the formation of a sandwich structure composed of AuNP-labeled tetanus antigen, tetanus antibody, and tetanus antigen. This leads to the formation of a characteristic red line due to the accumulation of AuNPs. The formation of the color line allows for a highly sensitive and selective detection of tetanus antibody, both with bare eyes and by smartphone-based quantitative analysis. This assay offers a wide detection range from 0 to 0.5 IU.mL(-1) and has a linear relationship from 0.01 to 0.1 IU.mL(-1) with an experimental detection limit of 0.01 IU.mL(-1). This assay is simple, fast, inexpensive and highly selective. When applied to the detection of tetanus antibody in spiked whole blood, it provided reliable results that compared well to those obtained with a commercial ELISA kit.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据