期刊
MICROCHEMICAL JOURNAL
卷 141, 期 -, 页码 247-252出版社
ELSEVIER
DOI: 10.1016/j.microc.2018.04.035
关键词
Ultrasonic assisted extraction; Physalis angulata; Phenolic compounds; Mixture design
资金
- Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologic (CNPq)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
The present article describes the development of an ultrasonic assisted extraction of phenolic compounds in Physalis angulata and determination of caffeic acid, rutin, catechin and trans-cinnamic acid by HPLC DAD. The mixtures were composed of extractor solvent and its ratio optimized using a mixture design. Doehlert matrix was used to optimize the extractor solvent volume and sonication time while using total phenolics as analytical response. The optimized conditions for the extractor solvent were the ratio of: 57% water, 35% ethanol and 8% methanol. The best experimental conditions for ultrasound were a sonication time of 10 min and extractor volume of 15 mL. The limit of detection varied from 5.0 to 30 mu g L-1. The methods precision was expressed as relative standard deviation (RSD) and presented values lower than 3.0% for all analytes. The accuracy was evaluated using spike test with recoveries between 91% and 107%. The method was applied for the determination of caffeic acid, rutin, catechin and trans-cinnamic in four samples of Physalis angulata aerial parts and the concentration varied from 15.5 to 22.5 mu g g(-1) for catechin, from 25.0 to 32.5 mu g g(-1) for caffeic acid, 75.6 to 88.2 mu g g(-1) for rutin and varied from 23.5 to 110.7 mu g g(-1) for trans-cinnamic acid.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据