期刊
MICROCHEMICAL JOURNAL
卷 141, 期 -, 页码 346-352出版社
ELSEVIER
DOI: 10.1016/j.microc.2018.05.045
关键词
Cholinium-amino acids ionic room temperature liquids (ChAARTILs); Multiwalled carbon nanotubes (MWCNTs); Trametes versicolor laccase (TvL); Immobilization strategy; Polyphenol index biosensor
资金
- Italian MIUR (Ministero dell'Istruzione, dell'Universita e della Ricerca) [000004_16_RS_DINI]
In this work, we present new cholinium-amino acids room temperature ionic liquids (ChAARTILs) that can be used as an efficient immobilization matrix for electrochemical biosensor development. The ideal immobilization strategy should be able to ensure the highest enzyme loading and a tight enzymatic immobilization, preserving its native structure and biological activity. In this regard, ChAARTILs present different side chains on the amino acids giving rise to van der Waals, pi-pi stacking and hydrogen bonding interactions. All these interactions can affect the nanomaterial organization onto the electrode surface. To this aim, we have evaluated the main electrochemical parameters, namely electroactive area (A(EA)) and the heterogeneous electron transfer rate constant (k(0)), showing how both cations and anions of room temperature ionic liquids (RTILs) can independently affect multi-walled carbon nanotubes (MWCNTs) organization. In particular, [Ch] [Phe] showed the best performance in terms of A(EA) (3.432 cm(2)) and k(0) (4.71.10(-3) cm s(-1)) with a homogeneous distribution of MWCNTs bundles onto the electrodes and a faster electron transfer rate. Finally, the modified electrode (MWCNTs-[Ch] [Phe]) has been tested with a model enzyme, namely Trametes versicolor laccase (Tvl), in order to evaluate the possibility to use ChAARTILs as immobilization matrix, preventing enzymatic denaturation phenomena which would affect the biosensor performance in terms of sensitivity, linear range, and stability.
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