期刊
MICROBIAL PATHOGENESIS
卷 123, 期 -, 页码 98-110出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2018.06.045
关键词
Fusarium; High-performance liquid chromatography; Liquid chromatography-electrospray ionization-tandem mass spectrometry; Matrix-assisted laser/desorption ionization time of flight mass spectrometry; Mycotoxins; Sugarcane
资金
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources [SB0704]
- KU Research Professor Program of Konkuk University, Seoul, Republic of Korea
- Guangxi special funding for distinguished experts
Mycotoxins are secondary metabolites of fungi that are damaging to both animals and humans. Extensive contamination of foods and feeds with mycotoxins is an important problem. Fumonisins, trichothecenes, zearalenone, and aflatoxins are mycotoxins produced by Fusarium species and occur naturally in sugarcane and cereal-based foods, threatening health and food security worldwide. Their distribution in the contaminated material is of great interest for obtaining insight into infection mechanisms and the potential for reducing contamination during food processing. In this study, mycotoxins were evaluated by high-performance liquid chromatography, liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and matrix-assisted laser/desorption ionization time of flight mass spectrometry (MALDI-TOF MS) of Fusarium species-infected sugarcane materials. A simple, sensitive, and reliable analytical method was developed for rapidly detecting eight mycotoxins in Fusarium species: fumonisin B1 and fumonisin B2, B-trichothecene mix (deoxynivalenol, nivalenol, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol), zearalenone, and aflatoxin G1. Analyses were carried out in multiple reaction monitoring mode using the two primary product ions. The results generated by LC/MS and MALDI-TOF MS/MS revealed various mechanisms regulating mycotoxins production, which may help to clarify the roles of sensitive and selective compounds. The results demonstrate that this procedure is suitable for simultaneous determination of mycotoxins in sugarcane and can be performed in routine analysis in mycotoxin laboratories.
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