期刊
MICROBIAL PATHOGENESIS
卷 117, 期 -, 页码 335-340出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2018.03.002
关键词
Vibrio; Vibriolysin-like proteases (VLPs); Phylogenetic tree; Classification; Structure; Vibrio pathogenicity
资金
- National Natural Science Foundation of China [31370104, 21205142, 31400002]
- Opening Foundation of the Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution [2015CNERC-CTHMP-07]
- Fundamental Research Funds for the Central Universities of Central South University [2016zzts583, 2016zzts164, 2017zzts076, 2017zzts351]
Vibriolysin-like proteases (VLPs) are important virulence agents in the arsenal of Vibrio causing instant cytotoxic effects during infection. Most of Vibrio secreted VLPs show serious pathogenicity, while some species of Vibrio with VLPs are non-pathogenic, like Vibrio tasmaniensis and Vibrio pacinii. To investigate the relation between VLPs and Vibrio pathogenicity, one phylogenetic tree of VLPs was constructed and compared consensus sequences at the N-terminus of VLPs. Based on these results, VLPs were defined into nine phylogenetic clades. Pathogenicity analysis of Vibrio showed that Vibrio species with VLPs III, VI, VII or VIII are serious pathogenic bacteria, while species with VLPs I, II, IV or IX are opportunistic pathogens. Multiple sequence alignment showed that the N-terminal 5-16 nucleotides of each Glade are highly conservative. Topological analysis of VLPs exhibited the structural differences in N-terminal regions of each VLP Glade. These results suggest that structure of N-terminus might play a key role in the pathogenicity of VLPs. Our findings give new insights into the classification of VLPs and the relationship between VLPs and Vibrio pathogenicity.
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