Study on Modified PVA- H3BO3 Immobilization Microorganism Method for Hydrogen Production from Wastewater

A new immobilization microorganism (IM) method was built by adding sodium alginate, SiO2 and CaCO3 in gel and cross-linking with saturated H3BO3 aqueous solution with 2% CaCl2 for traditional PVA-H3BO3 method. The modified method was used for preparation IM for hydrogen production from waste water contained organics by sewage treatment plants' sludge pretreated. The change rate of the IM balls diameter and unit hydrogen production were taken as the primary performance criterion of the IM. The modified IM method for hydrogen production from waste water contained organics was confirmed: 9% PVA and 0.9% sodium alginate for the embedding medium, saturated H3BO3 aqueous solution and 2% CaCl2 for cross-linking agent, and adding NaCO3 adjusting PH, 3%SiO2 and 0.5%CaCO3 for the support packing of IM balls, and the balls diameter of about 3mm. The modified IM balls had unit hydrogen production of 63.3% and total sugar removal rate of 143.4mL/h.L for washing model wastewater from ice cream factory, which contained 2000 mg/L total sugar and 5500mg/L COD, and higher mechanical strength. It were identified that the method could reduce outside surface's shrink, and improve the homogeneous of inside endoporus structure of modified IM balls, and a similar inside microporosity and outside microporosity by SEM detection.