期刊
MEDIATORS OF INFLAMMATION
卷 2018, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2018/3069521
关键词
-
资金
- Shandong Province Medical Science and Technology Development Project of China [2016WS0045]
- Key Research and Development Program of Shandong Province [2016GSF201021]
- Science and Technology Program of Higher Education of Shandong Province [J16LL01]
Purpose. To evaluate the regulating effect of Notch1 signaling on Th17/Treg immune imbalance in psoriasis vulgaris (PV). Materials and Methods. Notch1, Hes-1, ROR gamma t, Foxp3, IL-17, and IL-10 mRNA expression, as well as Th17 and Treg cell percentages in peripheral CD4(+) T cells, were detected by real-time quantitative RT-PCR and flow cytometry, and serum concentrations of IL-17 and IL-10 were detected by ELISA in 36 PV patients and 32 healthy controls. Additionally, CD4(+) T cells from 12 PV patients were treated with.-secretase inhibitor DAPT, and the above indexes were measured. Results. PV patients presented distinct Th17/Treg immune imbalance and highly expressed Notch1 and Hes-1 mRNA levels, which were positively correlated with psoriasis area and severity index (PASI) and the ratios of Th17/Treg and ROR gamma t/Foxp3. DAPT treatment resulted in the obvious downregulation of Th17 cell percentage in cocultured CD4(+) T cells, ROR gamma t and IL-17 mRNA levels, and IL-17 concentration in cell-free supernatant from cocultured CD4(+) T cells of PV patients in a dose-dependent manner, while there was no significant influence on Treg cell percentage, Foxp3, and IL-10 expression, therefore leading to the recovery of Th17/Treg immune imbalance. Conclusion. Notch1 signaling may contribute to the pathogenesis of PV by regulating Th17/Treg immune imbalance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据