期刊
MATERIALS TODAY
卷 21, 期 7, 页码 703-712出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mattod.2018.03.002
关键词
-
资金
- NSF Stem Cell Biomanufacturing IGERT
- Wallace H. Coulter Translational Partnership Research Award
- Achievement Rewards for College Scientists (ARCS) Scholars Award
- NIH [1R21CA191243-01A1]
- NSF Graduate Research Fellowship [DGE-1650044]
Efficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering and other biomedical applications. We discovered a unique cell biophysical phenomenon of transient cell volume exchange using microfluidics to rapidly and repeatedly compress cells. This behavior consists of brief, mechanically induced cell volume loss followed by rapid volume recovery. We harness this behavior for high-throughput, convective intracellular delivery of large polysaccharides (2000 kDa), particles (100 nm), and plasmids while maintaining high cell viability. Successful proof of concept experiments in transfection and intracellular labeling demonstrated potential to overcome the most prohibitive challenges in intracellular delivery for cell engineering.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据