期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 91, 期 -, 页码 236-246出版社
ELSEVIER
DOI: 10.1016/j.msec.2018.04.101
关键词
Hydrogels; Collagen; Alginate; Fibrin; Soft tissue engineering
资金
- EPSRC Re-distributed Manufacturing in Healthcare Network (RiHN) [EP/M017559/1]
- EPSRC Centre for Innovative Manufacture in Medical Devices (MeDe Innovation) [EP/K029592/1]
- Arthritis Research UK Tissue Engineering Centre [19429]
- EPSRC [EP/M017559/1, EP/K029592/1] Funding Source: UKRI
Hydrogels based on natural polymers offer a range of properties to mimic the native extracellular matrix, and provide microenvironments to preserve cellular function and encourage tissue formation. A tri-component hydrogel using collagen, alginate and fibrin (CAF) was developed and investigated at three collagen concentrations for application as a functional extracellular matrix analogue. Physical-chemical characterization of CAF hydrogels demonstrated a thermo-responsive crosslinking capacity at physiological conditions with stiffness similar to native soft tissues. CAF hydrogels were also assessed for cytocompatibility using L929 murine fibroblasts, pancreatic MIN6 beta-cells and human mesenchymal stem cells (hMSCs); and demonstrated good cell viability, proliferation and metabolic activity after 7 days of in vitro culture. CAF hydrogels, especially with 2.5% w/v collagen, increased alkaline phosphatase production in hMSCs indicating potential for the promotion of osteogenic activity. Moreover, CAF hydrogels also increased metabolic activity of MIN6 beta-cells and promoted the reconstitution of spherical pseudoislets with sizes ranging between 50 and 150 pm at day 7, demonstrating potential in diabetic therapeutic applications.
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