期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 88, 期 -, 页码 157-165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2018.03.009
关键词
Hyaluronic acid; 2-Thiophene ethylamine; Hepatocytes culture; RT-PCR; Immunostaining
资金
- Ministry of Science and Technology of China (MOST) [2016YFA0100500]
- National Natural Science Foundation of China (NSFC) [31571509, 31522038]
- ShanghaiTech University
Hyaluronic acid (HA) is a component of extracellular matrix, which is important for cell functions and tissue integrity. Biosynthesized HA, as well as its derivatives, is widely used in cosmetics industry, biochemical medicine and medical surgery. In this research, we report a new hyaluronic acid derivative synthesized by amidation of hyaluronic acid with 2-thiophene ethylamine (2TEA). 2-chloro-dimethoxy-1,3,5-triazine (CDMT) served as the activating agent of the carboxylic groups. Primary mouse hepatocytes cultured with this derivative HA-2TEA maintained their epithelial morphology and showed better hepatic functions. This result was confirmed by the higher expression levels of hepatic functional genes in primary hepatocyte cultured with HA-2TEA derivative. Moreover, the protein levels of several hepatic genes were further confirmed by immunofluorescence staining. Thus HA-2TEA(2-thiopheneethylamine) derivative demonstrated good capacity on hepatocytes culture, and maintained hepatocyte functions in vitro.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据