期刊
MARINE GENOMICS
卷 41, 期 -, 页码 54-56出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.margen.2018.03.004
关键词
Colwellia hornerae; Cold-active enzyme; Thermolabile industrial enzymes
资金
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT (MSIT) [NRF-2017M1A5A1013568]
- KOPRI's basic research project [PN17083, PN18082]
- National Research Foundation of Korea [2017M1A5A1013568] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Psychrophilic bacteria are considered a source of cold-active enzymes that can be used in industrial applications. The Arctic bacterium Colwellia hornerae PAMC 20917 strain has been isolated from the offshore sediment near Ny-Alesund, Svalbard. The optimal growth temperature of the strain was 10 degrees C on marine agar. The cell lysate showed alkaline phosphatase activities. Analysis of the enzymatic properties showed that the alkaline phosphatase was cold-active and thermolabile. To explore useful cold-active industrial enzymes further, the entire genome of the PAMC 20917 strain was sequenced. The genome of the strain contained 4,684,314 nucleotides, with 37.87% G + C content. Genome mining analysis revealed that, in the complete genome sequence, three proteins were annotated as alkaline phosphatases. The genome of PAMC 20917 encodes cold shock proteins and an ice-binding protein that inhibits the growth of ice, allowing the bacterium to adapt to cold environments. This genome information may be useful for understanding mechanisms of adaptation to cold stress.
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