期刊
MARINE DRUGS
卷 16, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/md16060190
关键词
Eustigmatos cf. polyphem; violaxanthin; culture conditions; purification; antioxidant activity
资金
- National High Technology Research and Development Program of China (863 Program) [2013AA065805]
- Special Program for Low-Carbon, Reform and Development Commission of Guangdong Province [2011-051]
- Key Program of Zhouhai City [PC20081008]
Violaxanthin is a major xanthophyll pigment in the microalga Eustigmatos cf. polyphem, but the amount produced after propagation can vary depending upon culture conditions. In this study, the effects of cultivation time, nitrogen concentration, light intensity, and culture mode on violaxanthin production were investigated. The results showed that this microalga vigorously grew and maintained a high level of violaxanthin in the fed-batch culture, and the highest violaxanthin productivity of 1.10 +/- 0.03 mg L-1 d(-1) was obtained under low light illumination with 18 mM of initial nitrogen supply for ten days. Additionally, violaxanthin was purified from E. cf. polyphem by silica gel chromatography and preparative high-performance liquid chromatography (PHPLC), and identified with high-resolution mass spectrometry (HRMS). The antioxidant activity of the purified violaxanthin was evaluated by three tests in vitro: reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assays. The strongest inhibition of purified violaxanthin occurred during the scavenging of ABTS(+) radicals, with EC50 of 15.25 mu g mL(-1). In conclusion, this is the first report to investigate the effects of different culture conditions on violaxanthin accumulation in E. cf. polyphem and provide a novel source for the production of violaxanthin that can be used for food and pharmaceutical applications.
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