期刊
ENDOCRINE
卷 44, 期 3, 页码 790-802出版社
HUMANA PRESS INC
DOI: 10.1007/s12020-013-9962-6
关键词
Tryptophan; Serotonin depletion; Physical exercise; Bone metabolism; Wnt pathway
资金
- FIRST, Italian Ministry of Education, University and Research
Several studies support a serotonin role in the physiological control of bone mass. However, whether serotonin (5-HT) is involved in bone loss due to reduced mechanical stress or unloading is unknown. We investigated the effects of reduced 5-HT tone, induced by tryptophan-free diet, in movement-restraint osteopenia induced by housing mature rats, acclimatised in single cages with a floor area of 1,500 cm(2), in smaller size single cages where their motor activity was reduced. Tryptophan-deficiency significantly worsened movement-restraint-induced bone loss in both femoral metaphysis and diaphysis (DXA analysis) but not at lumbar vertebrae and impaired the mechanical properties of the femur by significantly reducing both cortical thickness and strength strain index (pQCT analysis). Such effects resulted from an impairment of bone turnover with bone resorption exceeding bone formation. Tryptophan-supplemented diet reversed the worsening effects of tryptophan-deficiency on movement-restraint osteopenia. The improvements of both bone mass and strength were associated with an increase of serum osteocalcin and IGF-I, markers of osteoblast activity. In vitro studies in primary cultures of rat osteoblasts suggest that the anabolic action of 5-HT involves the activation of the Wnt/beta-catenin pathway. Serotonin significantly increased the cytoplasmatic beta-catenin protein levels by the inhibition of the enzyme glycogen synthase kinase-3 beta, that by phosphorylating beta-catenin promotes its degradation. Our data support a role for 5-HT in the anabolic response of the appendicular skeleton to mechanical loading. We suggest that serotonin might stimulate canonical Wnt/beta-catenin-dependent bone formation to occur.
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