Survival of probiotic bacteria in carboxymethyl cellulose-based edible film and assessment of quality parameters

In this study, survival of four probiotic strains (Lactobacillus acidophilus, L. casei, L. rhamnosus and Bifidobacterium bifidum) immobilized in edible films based on carboxymethyl cellulose (CMC) and physicochemical properties of films were investigated during 42 days of storage at 4 and 25 degrees C. Results showed a significant decrease in viability of bacterial cells during 42 days of storage at 25 degrees C. However, viability of L. acidophilus and L. rhamnosus were in the range of recommended levels during the storage at 4 degrees C (10(7) CFU/g). Probiotic films caused more water vapor permeability (WVP)and opacity, and less tensile strength (TS) and elongation at break (EB) compared to the control film. However, no significant physicochemical changes were observed among probiotic films containing different strains. Therefore, incorporation of some probiotic strains in edible coats and films could be their suitable carrier at refrigerated temperatures. (C) 2017 Elsevier Ltd. All rights reserved.