期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 97, 期 -, 页码 9-16出版社
ELSEVIER
DOI: 10.1016/j.lwt.2018.06.027
关键词
Chitosanase; Chitooligosaccharides; Glycoside hydrolase family 46; Enzyme membrane reactor; Product selectivity
资金
- National Natural Science Foundation of China [31701537]
- China Postdoctoral Science Foundation [2016M601530]
- Shanghai Sailing Program [17YF1403500]
- Fundamental Research Funds for the Central Universities [222201814031]
- Shu Guang project of Shanghai Municipal Education Commission
- Shanghai Education Development Foundation [15SG28]
Chitooligosaccharides are widely used in functional food, medicaments and agriculture. Controllable conversion of chitosan to chitooligosaccharides with desired degree of polymerization (DP) is of great value. Herein, a novel glycoside hydrolase family 46 chitosanase (BaCsn46A) was cloned and expressed from Bacillus amyloliquefaciens. BaCsn46A exhibited good enzymatic properties with high specific activity (1031.2 U/mg) under optimal hydrolysis conditions of pH 6.0 and 50 degrees C. BaCsn46A is an endo-type chitosanase, which could be utilized to yield abundant chitooligosaccharides. Furthermore, the preparation of high degree of polymerization chitooligosaccharides (DP 5-10) was investigated by BaCsn46A in an enzymatic reactor with a subsequent series of membranes. The maximum concentration of total chitooligosaccharides (DP 2-10) was 28.11 g/L (93.70% yield). The proportion of high degree of polymerization chitooligosaccharides (DP 5-10) occupied 56.17% (w/w) of total chitooligosaccharides. This study provided a cleaner production process for the controllable preparation of chitooligosaccharides with specific degree of polymerization.
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