期刊
LIFE SCIENCES
卷 205, 期 -, 页码 18-25出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2018.05.013
关键词
O-GlcNAc; Hyperglycemia; Placental dysfunction
资金
- Fundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT) [151371/2014]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [23038009165/2013-48]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [471675/2013-0, 305823/2015-9]
Aims: Hyperglycemia increases glycosylation with O-linked N-acetyl-glucosamine (O-GlcNAc) contributing to placental dysfunction and fetal growth impairment. Our aim was to determine how O-GlcNAc levels are affected by hyperglycemia and the O-GlcNAc distribution in different placental regions. Main methods: Female Wistar rats were divided into the following groups: severe hyperglycemia (> 300 mg/dL; n = 5); mild hyperglycemia (> 140 mg/dL, at least than two time points during oral glucose tolerance test; n = 7) or normoglycemia (< 120 mg/dL; n= 6). At 21 days of pregnancy, placental tissue was collected and processed for morphometry and immunohistochemistry analyses, or properly stored at -80 degrees C for protein quantification by western blot. Key findings: Placental index was increased only in severe hyperglycemic rats. Morphometric analysis showed increased junctional zone and decreased labyrinth region in placentas exclusively from the severe hyperglycemic group. Proteins targeted by O-GlcNAc were detected in all regions, with increased O-GlcNAc levels in the hyperglycemic group compared to control and mild hyperglycemic rats. Proteins in endothelial and trophoblast cells were the main target for O-GlcNAc. Whereas no changes in O-GlcNAc transferase (OGT) expression were detected, O-GlcNAcase (OGA) expression was reduced in placentas from the severe hyperglycemic group and augmented in placentas from the mild hyperglycemic group, compared with their respective control groups. Significance: Placental O-GlcNAc overexpression may contribute to placental dysfunction, as indicated by the placental index. Additionally, morphometric alterations, occurring simultaneously with increased O-GlcNAc accumulation in the placental tissue may contribute to placental dysfunction during hyperglycemia.
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