期刊
LIFE SCIENCES
卷 203, 期 -, 页码 112-120出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2018.04.031
关键词
Slo1; Docosahexaenoic acid; DHA; Sepsis; Immunonutrition; Mouse models; Blood pressure
资金
- German Research Foundation (DFG) [FOR1738, BA1601/8-2, CO912/2-1, HE2993/12]
- Federal Ministry of Education and Research (BMBF)
- Federal Ministry of Education and Research (Centre for Sepsis Control and Care) [FZK: 01EO1002]
- Federal Ministry of Education and Research (ZIK Septomics Research Centre, Translational Septomics) [FZK: 03Z22JN12]
- Federal Ministry of Education and Research (Competence Cluster for Nutrition and Cardiovascular Health, NutriCARD) [FZK: 01EA1411A]
Aims: Studies on omega-3 fatty acids, including docosahexaenoic acid (DHA), reveal diverging results: Their intake is recommended in cardiovascular disease and major surgery, while evidence argues against use in septic patients. DHA mediates its blood-pressure-lowering effect through Slo1 channels that are expressed on cardiovascular and immune cells. We hypothesised that conflicting effects of immunonutrition could be explained by the influence of omega-3 fatty acids on systemic blood pressure or immune effector cells through Slo1. Main methods: The effect of DHA on blood pressure was analysed in septic wild-type (WT) mice. Septic WT and Slo1 knockout (KO) mice were compared regarding survival, clinical presentation, haematology, cytokine release and bacterial burden. Cytokine expression and release of bone marrow derived macrophages (BMDM) from WT and Slo1 KO mice was assessed in response to LPS. Key findings: The significant blood-pressure-lowering effect of DHA in healthy animals was blunted in already hypotensive septic mice. Septic Slo1 KO mice displayed moderately lower bacterial burden in blood and lungs compared with WT, which did not translate into improved survival. Slo1 KO BMDM presented lower IL-6 levels in response to LPS, an effect that was abolished in the presence of DHA. More importantly, the strong inhibitory effect of DHA on IL-6 release was also observed in Slo1 KO BMDM. Significance: The controversial effects of immunonutrition in sepsis are unlikely to be primarily explained by the influence of DHA on blood pressure or effects on immune response mediated through Slo1 channels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据