期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 67, 期 3, 页码 235-243出版社
WILEY
DOI: 10.1111/lam.13015
关键词
acidic pH; biofilm; bovine mastitis; multidrug resistance; Streptococcus agalactiae
资金
- Fundacao de Amparoa Pesquisa do Estado, do Rio de Janeiro (FAPERJ)
- Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior (CAPES)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)
- Sub Reitoria de Pos-Graduacao e Pesquisa da Universidade do Estado do Rio de Janeiro (SR-2/UERJ)
Streptococcus agalactiae is among the most relevant aetiologic agent of bovine clinical and subclinical mastitis, a major problem for the dairy industry. In Brazil, clonal diversity, capsular typing and multidrug resistance profiles of S. agalactiae related to human and bovine infections need further investigation. Presently, S. agalactiae isolates of bovine subclinical mastitis, from Brazilian Northeastern region, were submitted to capsular and pulsed-field gel electrophoresis (PFGE)-typing, antimicrobial susceptibility and assays of biofilm formation at different time incubation and pH levels. Sixteen bovine isolates were characterized by polymerase chain reaction assay as S. agalactiae capsular type II (CTII) and classified by PFGE in A1/A2 (n=06), B1/B2 (n=06), C (n=03) and D (n=01) patterns. Bovine S. agalactiae CTII strains were classified as 25% multidrug-resistant (MDR) with susceptibility to penicillin, linezolid and vancomycin. Biofilm formation on abiotic surface was strain- and time-dependent with significantly higher rates at pH 65. In conclusion, S. agalactiae capsular type II isolates recovered from bovine subclinical mastitis produced different pH-dependent biofilm levels. Our findings suggest that biofilm production is modulated by environmental factors and provides S. agalactiae advantageous in colonizing mammary gland during mastitis development, including MDR strains.
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