期刊
LANGMUIR
卷 34, 期 17, 页码 5011-5019出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.8b00377
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资金
- Australian Research Council
- Queensland Government
The insulin immobilization behaviors of silica vesicles (SV) before and after modification with hydrophobic alkyl -C-8 and -C-18 groups have been studied and correlated to the grafted alkyl chain length. In order to minimize the influence from the other structural parameters, monolayered -C-8 or -C-18 groups are grafted onto SV with controlled density. The insulin immobilization capacity of SV is dependent on the initial insulin concentrations (IIC). At high IIC (2.6-3.0 mg/mL), the trend of insulin immobilization capacity of SV is SV-OH > SV-C-8 > SV-C-18, which is determined mainly by the surface area of SV. At medium IIC (0.6-1.9 mg/mL), the trend changes to SV-C-8 >= SV-C-18 > SV-OH as both the surface area and alkyl chain length contribute to the insulin immobilization. At an extremely low IIC, the hydrophobic hydrophobic interaction between the alkyl group and insulin molecules plays the most significant role. Consequently, SV-C-18 with longer alkyl groups and the highest hydrophobicity show the best insulin enrichment performance compared to SV-C-8 and SV-OH, as evidenced by an insulin detection limit of 0.001 ng/mL in phosphate buffered saline (PBS) and 0.05 ng/mL in artficial urine determined by mass spectrometry (MS).
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