期刊
LANGMUIR
卷 34, 期 4, 页码 1528-1534出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.7b03311
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资金
- excellence cluster The Hamburg Centre for Ultrafast Imaging - Structure, Dynamics and Control of Matter at the Atomic Scale of the Deutsche Forschungsgemeinschaft [EXC-1024]
Here we present a designer's approach to building cellular neuronal networks based on a biocompatible negative photoresist with embedded coaxial feedthroughs made of semiconductor microtubes. The diameter of the microtubes is tailored and adjusted to the diameter of cerebellum axons having a diameter of 2-3 mu m. The microtubes as well as the SU-8 layer serve as a topographical cue to the axons. Apart from the topographical guidance, we also employ chemical guidance cues enhancing neuron growth at designed spots. Therefore, the amino acid poly-L-lysine is printed in droplets of pl volume in the front of the tube entrances. Our artificial neuronal network has an extremely high yield of 85% of the somas settled at the desired locations. We complete this by basic patch-clamp measurements on single cells within the neuronal network.
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