期刊
JOURNAL OF APICULTURAL SCIENCE
卷 57, 期 2, 页码 93-100出版社
RESEARCH INST POMOLOGY FLORICULTURE, DIV APICULTURE
DOI: 10.2478/JAS-2013-0020
关键词
body composition; carbohydrates; enzymes of carbohydrates metabolism; lipids; proteins; Varroa destructor
类别
资金
- Ministry of Science and Higher Education, Poland [N N308 169338]
The aim of the present research was to determine the basic composition of the organic compounds present in extracts from Varroa destructor, a parasitic mite of the honeybee. The total protein content was 9.16 +/- 0.82 mg/100 mg of body weight, lipid content was 9.81 +/- 1.99 mg/100 mg, and carbohydrate content was 26.67 +/- 4.52 mg/100 mg. The triacylglycerol content was 2.40 +/- 0.86 mg/100 mg and the cholesterol content was 0.14 +/- 0.02 mg/100 mg. Thin layer chromatography indicated that phospholipids comprised the major part of the lipid component; cephalins (78%), cerebrosides (16%), and lecithins (6%) were identified in the phospholipid pool. Glucose (23.6 +/- 4.52 mg/100 mg) was the main carbohydrate, followed by glycogen (5.43 +/- 1.23 mg/100 mg) and trehalose (0.35 +/- 0.07 mg/100 mg). Enzyme-linked immunosorbent assays detected two major glycogen metabolism enzymes, glycogen phosphorylase and glycogen synthase. Among the enzymes metabolising disaccharides, maltase (24.7 +/- 2.38 mu mol/mg protein) and trehalase (14.81 +/- 5.21 mu mol/mg protein) presented the highest activity. Saccharose and lactose were hydrolysed to a minor extent. These are the first measurements of the basic composition of the mite body. Although these data are not exhaustive, they may serve as the basis for further research on the metabolism of V. destructor, particularly concerning lipid metabolism.
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