期刊
LAB ON A CHIP
卷 18, 期 6, 页码 869-877出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7lc01217a
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资金
- Novo Nordisk Foundation [NNF15OC0015246]
- Villum Fonden [9301]
- European Research Council [320535]
- Danish National Research Foundation [DNRF122]
- European Research Council (ERC) [320535] Funding Source: European Research Council (ERC)
- Novo Nordisk Fonden [NNF10CC1016517, NNF15OC0015246] Funding Source: researchfish
- Villum Fonden [00009301] Funding Source: researchfish
We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid-liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.
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