期刊
KIDNEY INTERNATIONAL
卷 93, 期 4, 页码 1008-1013出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.kint.2017.09.019
关键词
glomerulus; imaging; podocyte; renal pathology
资金
- Swedish Research Council [VR 2013-6041, 2015-04198, 2016-00968]
- Swedish Foundation for Strategic Research [RIF14-0091]
- Swedish Foundation for Strategic Research (SSF) [RIF14-0091] Funding Source: Swedish Foundation for Strategic Research (SSF)
- Swedish Research Council [2016-00968, 2015-04198] Funding Source: Swedish Research Council
The glomerular filtration barrier, has historically only been spatially resolved using electron microscopy due to the nanometer-scale dimensions of these structures. Recently, it was shown that the nanoscale distribution of proteins in the slit diaphragm can be resolved by fluorescence based stimulated emission depletion microscopy, in combination with optical clearing. Fluorescence microscopy has advantages over electron microscopy in terms of multiplex imaging of different epitopes, and also the amount of volumetric data that can be extracted from thicker samples. However, stimulated emission depletion microscopy is still a costly technique commonly not available to most life science researchers. An imaging technique with which the glomerular filtration barrier can be visualized using more standard fluorescence imaging techniques is thus desirable. Recent studies have shown that biological tissue samples can be isotropically expanded, revealing nanoscale localizations of multiple epitopes using confocal microscopy. Here we show that kidney samples can be expanded sufficiently to study the finest elements of the filtration barrier using confocal microscopy. Thus, our result opens up the possibility to study protein distributions and foot process morphology on the effective nanometer-scale.
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