期刊
KIDNEY INTERNATIONAL
卷 93, 期 1, 页码 69-80出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.kint.2017.04.031
关键词
apoptosis; diabetes; inflammation; renal pathology
资金
- National Key Research and Development Program [2016YFC0903400]
- National Science Foundation of China [81520108007, 31371272, 81500346, 81441401, 31471195]
- Science and Technology Development Project of Shandong Province [2014GSF118013]
- China Postdoctoral Science Foundation [2015M571997]
- Henry Ford Immunology Program Research Fund [T71015, T71016]
The urate oxidase (Uox) gene encodes uricase that in the rodent liver degrades uric acid into allantoin, forming an obstacle for establishing stable mouse models of hyperuricemia. The loss of uricase in humans during primate evolution causes their vulnerability to hyperuricemia. Thus, we generated a Uox-knockout mouse model on a pure C57BL/6J background using the transcription activator-like effector nuclease (TALEN) technique. These Uox-knockout mice spontaneously developed hyperuricemia (over 420 mu mol/l) with about 40% survival up to 62 weeks. Renal dysfunction (elevated serum creatinine and blood urea nitrogen) and glomerular/tubular lesions were observed in these Uox-knockout mice. Male Uox-knockout mice developed glycol-metabolic disorders associated with compromised insulin secretion and elevated vulnerability to streptozotocin-induced diabetes, whereas female mice developed hypertension accompanied by aberrant lipo-metabolism. Urate-lowering drugs reduced serum uric acid and improved hyperuricemia-induced disorders. Thus, uricase knockout provides a suitable mouse model to investigate hyperuricemia and associated disorders mimicking the human condition, suggesting that hyperuricemia has a causal role in the development of metabolic disorders and hypertension.
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