期刊
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
卷 12, 期 4, 页码 E2085-E2098出版社
WILEY
DOI: 10.1002/term.2641
关键词
bone regeneration; degradation; in vivo; micro-CT; MRI; superparamagnetic iron oxides
类别
资金
- China Postdoctoral Foundation [2015M571647]
- National Natural Science Foundation (NSF) of China [81400486, 81771044, 81672130]
- NSF of Jiangsu Province [BK20140911]
- Jiangsu Medical Youth Talent [QNRC2016853]
- Post-doctoral Foundation of Jiangsu [1402044B]
- National Key Research Project [2016YFA0201704/2016YFA0201700]
- Key Military Medical Projects [AWS14C007]
- Innovation Environment and Platform Construction Program [Z1511000003150134]
- Priority Academic Program Development of Jiangsu Higher Education Institutions [2014-37]
- University of Maryland School of Dentistry
A main challenge for use of scaffolds in bone engineering involves non-invasive monitoring in vivo and enhanced bone regeneration. The tissue repair effect of superparamagnetic iron oxide nanoparticles (SPIONs) was demonstrated previously by our group. However, testing in vivo is needed to confirm in vitro results. Here, SPIONs loaded gelatin sponge (GS) was used as a scaffold (SPIONs-GS) and implanted in the incisor sockets of Sprague-Dawley rats. Incisor sockets filled with nothing and filled with GS served as controls. Rats were sacrificed at 2 and 4weeks. A significant decrease in the signal intensity of T2-weighted magnetic resonance imaging (MRI) in the SPIONs-GS group was noted. Changes in image intensity of scaffolds (indicating scaffold degradation and interaction with host tissues) could be visually monitored over time. Microcomputed tomography showed that the SPIONs-GS group had more newly formed bone (64.44 +/- 10.92 vs. 28.1 +/- 4.49, p < .0001) and a better preserved alveolar ridge than blank control group at 4 weeks (0.962 +/- 0.01 vs. 0.92 +/- 0.01, p < .0001). Histology confirmed imaging results, showing good consistency in new bone formation and scaffold degradation. The number of SPIONs decreased rapidly with time due to quick degradation of GS, whereas the number of endocytic SPIONs in cells increased with time. These residual SPIONs, together with newly formed bone, could be detected by MRI at 4weeks. Therefore, it was clear that SPIONs induced active osteogenesis. In conclusion, good visibility on MRI and enhanced regeneration of bone can be obtained by implanting SPIONs-GS in vivo without using an external magnetic field.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据