Ultrasound improving the physical stability of oil-in-water emulsions stabilized by almond proteins

BACKGROUNDVegetable proteins are increasingly used to stabilize oil-in-water (O/W) emulsions. However, emulsions are thermodynamically unstable. Recently, high-intensity ultrasound (US) has been used to enhance the stability of emulsions. For these reasons, and considering almond (Prunus dulcis L.) as a good source of high-quality proteins, the aim of this work was to investigate the effect of US treatment on the stability of pre-emulsification O/W emulsions coated with almond protein isolate (API). RESULTSThe influence of API concentration (0.25-2.0gL(-1)), ion strength (0-500mmolL(-1) NaCl), and pH (3.0-7.0) on the stability of US-treated emulsions was evaluated. US treatment (200-600W, 25kHz, 15min) led to a significant reduction in the particles size of droplets in emulsions, increased critical osmotic pressure and additional protein interfacial adsorption, and thus the formation of more stable emulsions. The more unfolded and random coil structures of the proteins were detected at higher US power, facilitating protein interfacial adsorption. Increasing API concentrations resulted in higher stability of US-treated emulsions against untreated counterparts. The US-treated emulsions were more resistant to salt than untreated samples. In the range from pH3.0 to7.0, US treatment also enhanced the physical stability of emulsions compared with untreated emulsions. CONCLUSIONUS technology could be applied to produce more stable O/W food emulsions stabilized by proteins. (c) 2018 Society of Chemical Industry