期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 29, 期 7, 页码 1456-1462出版社
AMER CHEMICAL SOC
DOI: 10.1007/s13361-018-1956-4
关键词
Vitamin D; Matrix-assisted laser desorption/ionization; Signal-to-noise ratio; Mass spectrometry; Serum
资金
- Alfried Krupp von Bohlen und Halbach-Stiftung
- German Science Foundation [DFG VO 1355/5-1]
- HOMFOR [T201000779]
LC-MS/MS is widely utilized today for quantification of vitamin D in biological fluids. Mass spectrometric assays for vitamin D require very careful method optimization for precise and interference-free, accurate analyses however. Here, we explore chemical derivatization and matrix-assisted laser desorption/ionization (MALDI) as a rapid alternative for quantitative measurement of 25-hydroxyvitamin D-3 in human serum, and compare it to results from LC-MS/MS. The method implemented an automated imaging step of each MALDI spot, to locate areas of high intensity, avoid sweet spot phenomena, and thus improve precision. There was no statistically significant difference in vitamin D quantification between the MALDI-MS/MS and LC-MS/MS: mean +/- standard deviation for MALDI-MS-29.4 +/- 10.3 ng/mL-versus LC-MS/MS-30.3 +/- 11.2 ng/mL (P = 0.128)-for the sum of the 25-hydroxyvitamin D epimers. The MALDI-based assay avoided time-consuming chromatographic separation steps and was thus much faster than the LC-MS/MS assay. It also consumed less sample, required no organic solvents, and was readily automated. In this proof-of-concept study, MALDI-MS readily demonstrated its potential for mass spectrometric quantification of vitamin D compounds in biological fluids.
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