期刊
BIOIMPACTS
卷 4, 期 4, 页码 191-198出版社
TABRIZ UNIV MEDICAL SCIENCES & HEALTH SERVICES
DOI: 10.15171/bi.2014.019
关键词
Dorema glabrum; Phenolic compounds; Cytotoxicity; Genotoxicity
资金
- Research Center for Pharmaceutical Nanotechnology (RCPN), Tabriz University of Medical Sciences [90011, 90/011/101/1]
Introduction: Dorema glabrum (Apiaceae) is a rare and monocarpic species distributed in Transcaucasia and North West of Iran. We aimed to explore anticancer potency of bioactive compounds from the seeds of Dorma glabrum. Methods: Methanol extract was subjected to phytochemical investigation using normal phase Sep-pak and reversed-phase HPLC, and cytotoxic effect of isolated compounds on CAOV-4 cell line was evaluated. Furthermore, Annexin V/PI staining and comet assay were used to study genotoxicity of compounds. Results: Diglucosyl caffeoyl ester (1), Glucopyranosylcaffeic acid (2) and skimmin (3), were identified. MTT cytotoxicity assay showed growth inhibition of CAOV-4 cells due to treatment with compunds (1), (2) and (3) with an IC50 of 99.7, 87.3 and 70.03 mu g/ml at 48 h, respectively. Annexin V-FITC/PI staining showed occurrence of early/late apoptosis in the (1)- treated cells, while (2)- and (3)- treated cells necrosis/late apoptosis was dominant event. Single/double strands DNA breakages were observed by comet assay in all treatments. Conclusion: This work provides sufficient information about anti-cancer properties of the diglucosyl caffeoyl ester from the seeds of D. glabrum.
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