期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 140, 期 31, 页码 9979-9985出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.8b05203
关键词
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资金
- National Natural Science Foundation of China [21434007, 91427304, 21574122, 51573175]
- Fundamental Research Funds for the Central Universities [WK3450000002, WK2060200017]
- Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry (Changchun Institute of Applied Chemistry, Chinese Academy of Sciences)
Versatile building blocks are essential for building complex and scaled-up DNA circuits. In this study, we propose a conceptually new scalable architecture called a junction substrate (J-substrate) that is linked by prepurified double-stranded DNA molecules. As a proof-of-concept, this novel type of substrate has been utilized to build multi-input DNA circuits, offering several advantages over the conventional substrate (referred to as a linear substrate, L-substrate). First, the J-substrate does not require long DNA strands, thus avoiding significant synthetic errors and costs. Second, the traditional PAGE purification method is technically facilitated to obtain high-purity substrates, whereby the initial leakage is effectively eliminated. Third, the asymptotic leakage is eliminated by introducing the junction. Finally, circuits with the optimized J-substrate architecture exhibit fast kinetics. We believe that the proposed architecture constitutes a sophisticated chassis for constructing complex circuits.
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