期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 140, 期 3, 页码 1011-1018出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b10783
关键词
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资金
- Chemistry-Biology Interface Training Grant [T32 GM070421]
- National Science Foundation Graduate Research Fellowship Program [NGE-1144245]
- Springborn Fellowship
- Alfred P. Sloan fellowship [FG-2017-8964]
- Neurotechnology for Memory and Cognition Award
- Roy J. Carver Charitable Trust (Muscatine, Iowa) [15-4521]
- National Science Foundation, Division of Biological Infrastructure [DBI-0100085]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM070421] Funding Source: NIH RePORTER
Photoacoustic (PA) imaging is an emerging imaging modality that utilizes optical excitation and acoustic detection to enable high resolution at centimeter depths. The development of activatable PA probes can expand the utility of this technology to allow for detection of specific stimuli within live-animal models. Herein, we report the design, development, and evaluation of a series of Acoustogenic Probe(s) for Nitric Oxide (APNO) for the ratiometric, analyte-specific detection of nitric oxide (NO) in vivo. The best probe in the series, APNO-5, rapidly responds to NO to form an N-nitroso product with a concomitant 91 nm hypsochromic shift. This property enables ratiometric PA imaging upon selective irradiation of APNO-5 and the corresponding product, tAPNO-S. Moreover, APNO-5 displays the requisite photophysical characteristics for in vivo PA imaging (e.g., high absorptivity, low quantum yield) as well as high biocompatibility, stability, and selectivity for NO over a variety of biologically relevant analytes. APNO-5 was successfully applied to the detection of endogenous NO in a murine lipopolysaccharide-induced inflammation model. Our studies show a 1.9-fold increase in PA signal at 680 run and a 1.3-fold ratiometric turn-on relative to a saline control.
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