期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 140, 期 24, 页码 7716-7722出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.8b04404
关键词
-
资金
- NSFC [21622504, 21521063, 21735001]
- Science and Technology Project of Hunan Province [2017RS3019]
- Hunan University Fund for Multidisciplinary Developing [2015JCA04]
Fluorescent dyes have enabled much progress in the broad range of biomedical fields. However, many commercially available dyes suffer from small Stokes shifts, resulting in poor signal-to-noise ratio and self-quenching on current microscope configurations. In this work, we have developed a general method to significantly increase the Stokes shifts of common fluorophores. By simply appending a 1,4-diethyl-decahydro-quinoxaline (DOJ moiety onto the conjugated structure, we introduced a vibronic backbone that could facilely expand the Stokes shifts, emission wavelength, and photostability of 11 different fluorophores by more than 3-fold. This generalizable method could significantly improve the imaging efficiency of commercial fluorophores. As a demonstration, we showed that the DQ derivative of hemicyanine generated 5-fold signal in mouse models over indocyanine green. Furthermore, the DQ-modified fluorophores could pair with their parent molecules to conduct one-excitation, multiple emission imaging, allowing us to study the cell behavior more robustly. This approach shows promise in generating dyes suitable for super-resolution microscopy and second window near-infrared imaging.
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