期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 140, 期 13, 页码 4499-4502出版社
AMER CHEMICAL SOC
DOI: 10.1021/jacs.8b01510
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资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) [21522505]
- National Natural Science Foundation of China [21522505]
Functionalization of a gold surface with DNA is often complicated by kinetic traps from unintended DNA base adsorption. Herein, we communicate that BC serves as a robust backfilling agent displacing selected DNA bases on gold. Traditional thiol backfillers are too strong, while even 300 mM BC is well tolerated. Conjugates prepared with BC hybridize 10-fold faster and resist DNA release with better colloidal stability yielding highly sensitive probes. From colorimetric and Raman assays, adsorption affinity ranks as F- < T approximate to Cl- < C < G approximate to BR- < A < I-, allowing Br- to displace nonpoly-A sequences from gold. This well-controlled biointerface will impact biosensing, drug delivery, and directed assembly of nanomaterials.
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