期刊
JOURNAL OF STORED PRODUCTS RESEARCH
卷 77, 期 -, 页码 156-165出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jspr.2018.04.012
关键词
Lasioderma serricorne; beta-N-acetylglucosaminidase; Development; 20-hydroxyecdysone; RNA interference
类别
资金
- National Natural Science Foundation of China [31501649]
- Program for Science & Technology Outstanding talents in Higher Learning Institution of Guizhou Province [2017083]
- Program for First-class Discipline Construction in Guizhou Province [201785]
- Scientific Research Foundation for Talent Introduced in Guizhou University [201668]
- Engineering Research Center of Pest Ecological Management in Guizhou Province [1491]
beta-N-acetylglucosaminidases (NAGs) are essential enzymes in the chitin degradation process and play important roles in insect molting and metamorphosis. In this study, we identified and characterized a full-length cDNA of NAG1 (LsNAG1) in the cigarette beetle, Lasioderma serricorne (Fabricius). The open reading frame of LsNAG1 was 1821 bp and encoded 606 amino acid residues that contained typical domain structure of NAGs. Phylogenetic analyses suggested that the deduced protein LsNAG1 was orthologous to another insect group I NAGs. Quantitative real-time PCR confirmed that LsNAG1 expression levels peaked periodically in the late larval and late pupal stages. A significantly increased level of LsNAG1 transcripts was observed in larvae exposed to the steroid hormone 20-hydroxyecdysone (20E), indicating LsNAG1 was responsive to 20E. Injection of double-stranded RNA (dsRNA) of LsNAG1 into 4th instar larvae and late pupae significantly reduced LsNAG1 transcript levels in both stages, and led to severe molting defects and high mortality. Compared with the control insects injected with dsGFP, 48% and 83% of the larvae and pupae injected with dsLsNAG1, respectively, died during or soon after molting to the next stage. These results demonstrate that LsNAG1 may play a crucial role in successful larval pupal and pupal adult transitions, and could be used as an efficient novel target for storage pest control. (C) 2018 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据