期刊
JOURNAL OF SEPARATION SCIENCE
卷 41, 期 13, 页码 2767-2773出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201701417
关键词
glycoproteins; graphene oxide; monolithic columns; solid-phase microextraction
资金
- National Natural Science Foundation of China [81573384, 21375101]
A poly(vinylphenylboronic acid-ethylene glycol dimethacrylate) monolithic material incorporated with graphene oxide was synthesized inside a poly(ether ether ketone) tube. This tube with boronate affinity monolith was coupled with a high-performance liquid chromatography system through a six-port valve to construct an online solid-phase microextraction with high-performance liquid chromatography system. The performance of this solid-phase microextraction with high-performance liquid chromatography system was demonstrated by standard glycoprotein in aqueous samples, namely, horseradish peroxidase. Some parameters that affect the extraction performance were investigated, including sampling rate, pH of sample solution, and sampling volume. Under the optimized conditions, the developed method showed high extraction efficiency toward horseradish peroxidase. The addition of graphene oxide greatly increased the extraction efficiency of boronate affinity monolith for horseradish peroxidase. The limit of detection of the proposed method was as low as 0.01 mu g/mL by using ultraviolet detection. The recognition specificity was also evaluated by analyzing the mixture of bovine serum albumin (nonglycoprotein) and horseradish peroxidase. The results showed that this material could selectively extract horseradish peroxidase from the mixture, indicating its good specificity toward glycoproteins. The proposed method was further applied for analyzing rat plasma samples spiked with horseradish peroxidase. Good recovery and repeatability were obtained.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据