期刊
JOURNAL OF SEPARATION SCIENCE
卷 41, 期 13, 页码 2743-2749出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201800175
关键词
biomarkers; in-tube solid-phase microextraction; liquid chromatography; reactive oxygen species; tandem mass spectrometry
资金
- [26460053]
This study described an automated online method for the simultaneous determination of 8-isoprostane, 8-hydroxy-2'-deoxyguanosine, and 3-nitro-L-tyrosine in human urine. The method involves in-tube solid-phase microextraction using a Carboxen 1006 PLOT capillary column as an extraction device, followed by liquid chromatography with tandem mass spectrometry using a CX column and detection in the negative/positive switching ion-mode by multiple reaction monitoring. Using their stable isotope-labeled internal standards, each of these oxidative stress biomarkers showed good linearity from 0.02 to 2.0 ng/mL. Their detection limits (S/N = 3) were 3.421.5 pg/mL, and their intra- and inter-day precisions (relative standard deviations) were >3.9 and 6.5% (n = 5), respectively. This method was applied successfully to the analysis of urine samples, without any other pretreatment and interference peaks.
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