期刊
JOURNAL OF SEPARATION SCIENCE
卷 41, 期 16, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201800458
关键词
biomarkers; human urine; l-kynurenine; molecularly imprinted polymers; solid-phase extraction
l-Kynurenine is an endogenous metabolite generated by the catabolic pathway of l-tryptophan and it could be a potential biomarker to test the efficacy of several checkpoint inhibitors that have already reached the clinical trials in the antitumor therapy. Thus, a molecularly imprinted polymer specific for the recognition of this metabolite was synthesized and used as innovative system in solid-phase extraction technique for the specific extraction and quantification of l-kynurenine in human urine. The off-line system was firstly tested on l-kynurenine standard solutions, allowing recoveries up to 97.7% (relative standard deviation=2.2%) and then applied to fortified and deproteinated human urine samples, where a recovery of 84.1% (relative standard deviation=3.1%) was obtained. The method was validated and it revealed a good linearity in the range of 0.157-20g/mL (r(2)=0.9992). The optimized procedure demonstrated a good feasibility on biological samples, allowing a ready quantification of l-kynurenine in the human urine, where the metabolite was found at a very low concentration (0.80g/mL). The extraction system developed could attract attention of pharmaceutical industries for l-kynurenine production as potential drug in the treatment of autoimmune disorders through its extraction and purification from biological matrixes.
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