期刊
JOURNAL OF SEPARATION SCIENCE
卷 41, 期 9, 页码 2064-2084出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201701411
关键词
miniaturized matrix solid-phase dispersion; quadrupole time-of-flight mass spectrometry; traditional Chinese medicine; ultra high performance liquid chromatography
资金
- Program of International S&T Cooperation Project of China [2015DFA30430]
- National Natural Science Foundation of China [81374050]
- National Science and Technology Support Program Projects [2014BA105B01]
A simple and effective sample preparation process based on miniaturized matrix solid-phase dispersion was developed for simultaneous determination of phenolic acids (gallic acid, chlorogenic acid, ferulic acid, 3,5-dicaffeoylqunic acid, 1,5-dicaffeoylqunie acid, rosmarinic acid, lithospermic acid, and salvianolic acid B), flavonoids (kaempferol-3-O-rutinoside, calycosin, and formononetin), lactones (ligustilide and butyllidephthalide), monoterpenoids (paeoniflorin), phenan-thraquinones (cryptotanshinone), and furans (5-hydroxymethylfurfural) in Naoxintong capsule by ultra high-performance liquid chromatography. The optimized condition was that 25 mg Naoxintong powder was blended homogeneously with 100 mg Florisil PR for 4 min. One milliliter of methanol/water (75:25, v/v) acidified by 0.05% formic acid was selected to elute all components. It was found that the recoveries of the six types of components ranged from 61.36 to 96.94%. The proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography was successfully applied to simultaneous determination of the six types of components in Naoxintong capsules. The results demonstrated that the proposed miniaturized matrix solid-phase dispersion coupled with ultra high-performance liquid chromatography could be used as an environmentally friendly tool for the extraction and determination of multiple bioactive components in natural products.
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