期刊
JOURNAL OF SEPARATION SCIENCE
卷 41, 期 16, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201800403
关键词
bioanalytical chemistry; clinical chemistry; electrochemical detection; portable instrumentation
资金
- CNPq [448089/2014-9, 308140/2016-8]
- CAPES [3363/2014]
- INCTBio [465389/2014-7]
- FAPEG [201210267000929]
- CNPq
- CAPES
We describe the assembly of a hybrid electrophoresis device that contains fused silica capillaries interconnected to a microfabricated interface in a cross format for the determination of inorganic cations in biological samples. The sample transport in the proposed hybrid device was performed under gated injection mode and the separations were monitored with a capacitively coupled contactless conductivity detector. The capillary extremities were inserted into polypropylene tubes to create solution reservoirs. Sensing electrodes were produced using stainless-steel hypodermic needles previously cut with 2.0mm length. The running composition and injection time were optimized and the best results were found using 50mmol/L lactic acid, 20mmol/L histidine and 3mmol/L 18-crown-6 ether, and an electrokinetic injection time of 15s. The separation of six inorganic cations was achieved with baseline resolution, and efficiencies were between 9.1x10(3) and 5.4x10(4) plates/m. The proposed hybrid device was explored for determining the concentration levels of inorganic cations in urine, saliva, and tear samples, employing Li+ as an internal standard. The achieved results were in good agreement with the data reported in the literature. The reliability of the proposed method ranged from 93 to 98%, thus suggesting satisfactory accuracy for bioanalytical applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据