期刊
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 125, 期 -, 页码 1-7出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jri.2017.10.046
关键词
Endometriosis; Complement system; Decay accelerating factor (DAF); Endometrial receptivity
资金
- Fondo Nacional de Desarrollo Cientifico y Tecnologico, FONDECYT, Chile [1140688]
- Eunice Kennedy Shriver National Institute Of Child Health & Human Development of the National Institutes of Health [R01HD067721]
- Canadian Institutes of Health Research
- Fogarty International Fellowship Award
The control of complement activation within embryo-endometrium environment is critical for embryo survival. Cell evasion from complement attack requires interaction of complement regulatory proteins (CRPs) with cell adhesion alpha v beta 3 integrin. We aim to compare the expression of CRPs in endometria of women with and without endometriosis and to examine the molecular interaction of decay accelerating factor (DAF) with alpha v beta 3 integrin. Endometrial expression of Membrane cofactor protein (CD46), Decay accelerating factor (DAF), Membrane attack complex inhibitory factor (CD59) and beta 3 integrin subunit were determined through menstrual cycle by immunohistochemistry. DAF protein quantity was determined by Western blot and mRNA levels measured in epithelial cells isolated by laser capture microdissection (LCM). Using in vitro assay, we examined DAF and beta 3 integrin expression through paracrine regulation between endometrial compartments. To determine whether beta 3 integrin and DAF interacts in vivo, endometrial samples were subjected to immunoprecipitation and colocalization using dual immunofluorescence technique. DAF and beta 3 integrin expression were significantly low in samples from women with endometriosis during mid secretory phase. This observation was supported by decreased DAF protein quantity; faint DAF and beta 3 integrin interaction and reduced mRNA levels in cells dissected by LCM. Moreover epithelial DAF and beta 3 integrin expression through paracrine regulation by progesterone from stromal compartment was disrupted in endometriosis. Endometria from women with endometriosis exhibits aberrant expression of complement proteins. The abnormal DAF expression potentially compromises embryo survival, contributing to understand the implantation failure in women with endometriosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据