期刊
JOURNAL OF REPRODUCTION AND DEVELOPMENT
卷 64, 期 3, 页码 243-251出版社
SOCIETY REPRODUCTION & DEVELOPMENT-SRD
DOI: 10.1262/jrd.2017-160
关键词
ATP content; Glutathione; Heat stress; Oxidative phosphorylation; Transcriptome
资金
- state of Tennessee through UT AgResearch, Department of Animal Science, East Tennessee Research and Education Center
- USDA National Institute of Food and Agriculture [227701]
- National Research Initiative Competitive Grant [35203-14772]
- Vanderbilt Ingram Cancer Center [PA CA68485]
Hyperthermia during estrus has direct consequences on the maturing oocyte that carries over to the resultant embryo to compromise its ability to continue in development. Because early embryonic development is reliant upon maternal transcripts and other ooplasmic components, we examined impact of heat stress on bovine oocyte transcripts using microarray. Oocytes were matured at 38.5 degrees C for 24 h or 41.0 degrees C for the first 12 h of in vitro maturation; 38.5 degrees C thereafter. Transcriptome profile was performed on total (adenylated + deadenylated) RNA and polyadenylated mRNA populations. Heat stress exposure altered the abundance of several transcripts important for mitochondrial function. The extent to which transcript differences are coincident with functional changes was evaluated by examining reactive oxygen species, ATP content, and glutathione levels. Mitochondrial reactive oxygen species levels were increased by 6 h exposure to 41.0 degrees C while cytoplasmic levels were reduced compared to controls (P < 0.0001). Exposure to 41.0 degrees C for 12 h increased total and reduced glutathione levels in oocytes at 12 h but reduced them by 24 h (time x temperature P < 0.001). ATP content was higher in heat-stressed oocytes at 24 h (P < 0.0001). Heat-induced increases in ATP content of matured oocytes persisted in early cleavage-stage embryos (8 to 16-cell embryos; P < 0.05) but were no longer apparent in blastocysts (P > 0.05). Collectively, results indicate that direct exposure of maturing oocytes to heat stress may alter oocyte mitochondrial processes/function, which is inherited by the early embryo after fertilization.
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