期刊
JOURNAL OF PROTEOMICS
卷 187, 期 -, 页码 59-68出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jprot.2018.06.003
关键词
Venom proteome; Taiwanese venomous snakes; Cobra venom factor; SISCAPA-MRM
资金
- Molecular Medicine Research Center, Chang Gung University from The Featured Areas Research Center Program within Ministry of Education (MOE) in Taiwan [EMRPD1H0171]
- Ministry of Science and Technology, Taiwan [MOST 106-2632-B-182-002, 106-2320-B-182-029-MY3]
- Chang Gung Memorial Hospital, Taiwan [CMRPG3F0241-243, CMRPD1B0531, CIRPD3B0013, CRRPD1F0051-52, CMRPD190364, CLRPD190018, BMRP208]
Deinagkistrodon acutus, Trimeresurus stejnegeri, Protobothrops mucrosquamatus, Daboia russelii siamensis, Bungarus multicinctus and Naja atra are the six medically important venomous snake species in Taiwan. In this study, we characterized and compared their venom protein profiles using proteomic approaches. The major snake venom proteins were identified by GeLC-MS/MS and the total venom proteome was characterized by in-solution digestion coupled with LC-MS/MS. A total of 27-52 proteins, categorized into 23 protein families, were identified in each snakes venom. The major venom components found in Viperidae species (D. acutus, T. stejnegeri, P. mucrosquamatus and D. russelii) were C-type lectin, snake venom serine proteinase, venom metalloproteinase and phospholipase A(2), whereas three-finger toxin and phospholipase A(2) were the major components detected in the venom of Elapidae snakes (B. multicinctus and N. atra). This study also provided the first demonstration of some low-abundance proteins in these six snake venoms, including 5'-nucleotidase, glutaminyl-peptide cyclotransferase and phosphodiesterase, among others. Furthermore, we found that cobra venom factor (CVF) is a cobra-specific protein. We produced anti-peptide antibodies against CVF and used it to develop a highly sensitive SISCAPA-MRM assay for quantifying CVF. The limit of detection and lower limit of quantification were 3.2 and 9.6 attomoles, respectively. This assay was used to precisely quantify CVF in 1 mu g crude venom proteins from three Naja species and king cobra. The amount of CVF varied from 0.9 to 54.36 femtomoles (equivalent to 0.16-10.03 mg/g of venom protein). Biological significance: There are six medically significant,venomous snakes in Taiwan. The venoms of the four Viperidae species (Deinagkistrodon acutus, Trimeresurus stejnegeri, Protobothrops mucrosquamatus and Daboia russelii siamensis) cause local tissue swelling; this symptom is also seen in N. atra envenomation in humans, potentially complicating the differential diagnosis of envenomation by N. atra and Viperidae species. Thus, characterization of the venom proteomes of the six Taiwanese snakes, including the relative abundance of the major components and species-specific protein(s) in each venom type, could be useful for future venom research, including the development of new assay(s) for detecting snake species-specific venom protein(s) and new type(s) of anti venom.
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