期刊
JOURNAL OF PLANT PATHOLOGY
卷 101, 期 1, 页码 39-49出版社
SPRINGER
DOI: 10.1007/s42161-018-0126-7
关键词
P; carotovorum subsp; brasiliense; Complete genome sequence; Effector
资金
- National Key R&D Program of China [2017YED0201100]
Pectobacterium carotovorum subsp. brasiliense (Pcb) is a gram-negative, plant pathogenic bacterium of the soft rot Enterobacteriaceae (SRE) family. We present the complete genome sequence of Pcb strain BZA12, which reveals that Pcb strain BZA12 carries a single 4,924,809bp chromosome with 51.97% GC content and comprises 4508 predicted protein-coding genes. Gene annotation of these genes utilized GO, KEGG, and COG databases. In comparison with three closely related soft-rot pathogens, strain BZA12 has 3797 gene families, among which 3107 gene families are identified as orthologous with those of both P. carotovorum subsp. carotovorum PCC21 and P. carotovorum subsp. odoriferum BCS7, as well as 36 putative Unique Gene Families. We selected five putative effectors from the BZA12 genome and transiently expressed them in Nicotiana benthamiana. Candidate effector A12GL002483 was localized in the cell nucleus and induced cell death. This study provides a foundation for a better understanding of the genomic structure and function of Pcb, particularly in the discovery of potential pathogenic factors and for the development of more effective strategies against this pathogen.
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