Role of fetal nutrient restriction and postnatal catch-up growth on structural and mechanical alterations of rat aorta

Fetal undernutrition programmes hypertension development, males being more susceptible. Deficient fetal elastogenesis and vascular growth is a possible mechanism. We investigated the role of aortic mechanical alterations in a rat model of hypertension programming, evaluating changes at birth, weaning and adulthood. Dams were fed ad libitum (Control) or 50% of control intake during the second half of gestation (maternal undernutrition, MUN). Offspring aged 3 days, 21 days and 6 months were studied. Blood pressure was evaluated in vivo. In the thoracic aorta we assessed gross structure, mechanical properties (intact and purified elastin), collagen and elastin content and internal elastic lamina (IEL) organization. Only adult MUN males developed hypertension (systolic blood pressure: MUNmales = 176.6 +/- 5.6 mmHg; Control(males) = 136.1 +/- 4.9 mmHg). At birth MUN rats were lighter, with smaller aortic cross-sectional area (MUNmales = (1.51 +/- 0.08) x 10(5) mu m(2), Control(males) = (2.8 +/- 0.04) x 10(5) mu m(2)); during lactation MUN males and females exhibited catch-up growth and aortic hypertrophy (MUNmales = (14.5 +/- 0.5) x 10(5) mu m(2), Control(males) = (10.4 +/- 0.9) x 10(5) mu m(2)), maintained until adulthood. MUN aortas were more compliant until weaning (functional stiffness: MUNmales = 1.0 +/- 0.04; Control(males) = 1.3 +/- 0.03), containing less collagen with larger IEL fenestrae, returning to normal in adulthood. Purified elastin from young MUN offspring was more compliant in both sexes; only MUN adult females maintained larger elastin compliance (slope: MUNfemales = 24.1 +/- 1.9; Control(females) = 33.3 +/- 2.8). Fetal undernutrition induces deficient aortic development followed by hypertrophic remodelling and larger aortic compliance in the perinatal period, with similar alterations in collagen and elastin in both sexes. The observed alterations argue against an initial mechanical cause for sex differences in hypertension development. However, the maintenance of high elastin compliance in adult females might protect them against blood pressure rise.